Site-specific analysis of gene expression in early osteoarthritis using the Pond-Nuki model in dogs

Table 3 Differentially expressed genes in the ACL-X knee by region in the tibial plateau

p value	ACL-X	Control	CrMTP	CrLTP	ACL-X	Control	p value
<0.05	0.093 ± 0.02	0.018 ± 0.018	MMP 13	COL 1	5.46 ± 3.96	0.48 ± 0.08	<0.05
				COL 2	1022 ± 651	254 ± 84	<0.05
				MMP 13	0.147 ± 0.012	0 ± 0	<0.05
				INOS	0.362 ± 0.065	0.04 ± 0.04	<0.05
				COX-2	0.007 ± 0.005	0 ± 0	<0.05
				TIMP-1	35.3 ± 4.6	8.4 ± 5.4	<0.05
				MMP 3	2.428 ± 1.272	0.587 ± 0.18	<0.05
				ADAMTS 5	0.132 ± 0.05	0.024 ± 0.024	<0.05
p value	ACL-X	Control	CaMTP	CaLTP	ACL-X	Control	p value
<0.05	378.6 ± 321.42	9.05 ± 7.08	COL 1	COL 1	78.85 ± 43.79	1.98 ± 1.72	<0.05
<0.05	2787 ± 1919	594 ± 151	COL 2	COL 2	1926 ± 939	708 ± 49	<0.05
<0.05	0.074 ± 0.059	0.007 ± 0.007	MMP 13	MMP 13	0.05 ± 0.002	0.011 ± 0.011	<0.05
<0.05	0.051 ± 0.044	0.003 ± 0.001	COX-2	COX-2	0.019 ± 0.006	0.002 ± 0.002	<0.05
<0.05	0.189 ± 0.019	0.088 ± 0.002	INOS
<0.05	0.023 ± 0.011	0.002 ± 0.002	ADAMTS 5
<0.05	1.4 ± 0.07	3.71 ± 0.08	TIMP-2	TIMP-2	0.99 ± 0.02	2.7 ± 0.8	<0.05

Differentially expressed genes in the ACL-transected knee by region in the tibial plateau compared to the contralateral normal control. All genes listed were up regulated in the ACL-transected stifle except TIMP-2, which was down regulated in the ACL-transected stifle. Values listed are the mean relative level of expression (± standard error) for each gene compared to the house keeping gene GAPDH. Significant differences were determined using REST-XL, and relative expression levels were determined using Q-Gene.

ISSN: 1749-799X