Figure 5
Osteobalsts DNA degradation induced by caffeine. Activation of an irreversible commitment to cell death by caffeine was clearly demonstrated in the DNA fragmentation analysis. The formation of DNA fragments of was easily observed when osteoblasts cultured with caffeine. Electrophoresis of genomic DNA from osteoblast cells that were exposed to 5.0 and 10 nM caffein showed the characteristic laddering pattern (in the size of 500 – 1000 bp) that led to cell death in the first day's culture; while in the concentrations of 0.5, 1.0 or 2.5 nM caffeine, the appearance of DAN fragmentation appeared at the 3rd day's culture with the characteristic laddering pattern in the size of 200 – 1000 bp.